Fig. 4

Normal cell proliferation in the Pdk1 cKO subpallium. (a-f′) Immunofluorescence for PH3 showed that the number of PH3-labeled M phase cells was unaffected in the MGE of Pdk1 cKO mice compared with control at E12.5, E14.5, and E16.5. (s) Statistical analysis of PH3⁺ cells (E12.5 Pdk1 cKO: N = 3, Ctrl: N = 3, P = 0.7922; E14.5 Pdk1 cKO: N = 3, Ctrl: N = 3, P > 0.9999; E16.5 Pdk1 cKO: N = 3, Ctrl: N = 3, P = 0.5767, Multiple t-test, with alpha = 0.05/3 = 0.0167). (g-l’) Immunofluorescence for BrdU showed that the number of S phase cells within the Pdk1 cKO MGE was similar to that in the control MGE at E12.5, E14.5, and E16.5. (t) Statistical analysis of BrdU⁺ cells (E12.5 Pdk1 cKO: N = 3, Ctrl: N = 3, P = 0.9502; E14.5 Pdk1 cKO: N = 3, Ctrl: N = 3, P = 0.9265; E16.5 Pdk1 cKO: N = 5, Ctrl: N = 5, P = 0.7189, Multiple t-test, with alpha = 0.05/3 = 0.0167). (m-r’) Immunofluorescence for Ki67 revealed comparable numbers of Ki67⁺ cells between Pdk1 cKO and control mice at E12.5, E14.5, and E16.5. (u) Statistical analysis of Ki67⁺ cells (E12.5 Pdk1 cKO: N = 3, Ctrl: N = 3, P = 0.9954; E14.5 Pdk1 cKO: N = 4, Ctrl: N = 4, P = 0.8033; E16.5 Pdk1 cKO: N = 6, Ctrl: N = 6, P = 0.9003, Multiple t-test, with alpha = 0.05/3 = 0.0167). The data are presented as the mean ± SEM. Scale bar, 100 μm