Fig. 4

Differentiation and population of Th17 cells was increased in the brains of CPMS mice. a Representative image of western blot analysis in the Hip, Amy, and PFC. b TGF-β (Hip; 2.288 ± 0.1537, Amy; 4.342 ± 0.4557, PFC; 1.757 ± 0.2707 vs CTL), and c pSTAT3/tSTAT3 (Hip; 4.563 ± 1.284, Amy; 4.850 ± 1.628, PFC; 4.278 ± 1.152 vs CTL) were normalized by GAPDH. d RORγt (Hip; 1.374 ± 0.1462, Amy; 1.700 ± 0.2548, PFC; 1.399 ± 0.215 vs CTL) was normalized by Lamin B1. e Flow cytometry was performed to detect CD4⁺ IL-17⁺ cells (CTL (n = 5); 0.0476 ± 0.006592, CPMS (n = 6); 0.1158 ± 0.01638) in whole brain of the 8-week-old mice. All data are presented as mean ± SEM of three or more independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. CTL