Table 1 Sequence of primers used for RT-PCR
Gene | Accession no. | Sequence of; F, forward primer; R, reverse primer | Region | Product |
|---|---|---|---|---|
β-actin | Rat | F:5′-ATGGTGGGTATGGGTCAGAAGG-3′ | 208–472 | 265 bp |
|  | NM-031144 | R: 5′-TGGCTGGGGTGTTGAAGGTC-3’ |  |  |
BKCaαsubunit | Rat | F: 5′- CAGCACTCCGCAGACACTA-3’ | 3774–3879 | 106 bp |
|  | NM-031828.1 | R:5′- ATCACCATAACAACCACCATCC-3’ |  |  |
BKCaβ1 subunit | Rat | F:5′-CGCCATCACTTACTACATCCT − 3’ | 394–682 | 289 bp |
|  | 019273.1 | R:5′-GACCTTCTTCACATCAACCAA-3’ |  |  |
BKCaβ2 subunit | Rat | F: 5′-CGGACCTCTTCATCTTACAGAC-3’ | 22–210 | 189 bp |
|  | 176,861.1 | R:5′-CAGCAGTGTGATTCCCAGTAG − 3’ |  |  |
BKCaβ3 subunit | Rat | F:5′-CGCTCTGATTGTTGGTTTGGT − 3’ | 899–1059 | 161 bp |
|  | 001104560.2 | R:5′-CTCTGTTCCTTCCCTGACTCTT-3’ |  |  |
BKCaβ4 subunit | Rat | F:5′-CATCCTGTCGCTCTTCATCTTC-3’ | 102–335 | 234 bp |
|  | 023960.2 | R: 5′ -TGGTGCTGGTCGCTGTGTA-3’ |  |  |