Fig. 4

Direct comparison of Ca²⁺ increase by double labeling. a Images of fresh cerebellar sagittal slices expressing dT (red) and mTB (blue) in different groups of GCs. Note that the dT- and mTB-positive PF bundles are located at different sublayers of the ML. The roman numerals indicate the lobes. b, c Difference in Ca²⁺ responses between the two groups of GCs in control (b, black, n = 32, 33, or 27 slices for M–D, S–M, or S–D, respectively), in the presence of bicuculline (b, blue, n = 17, 18, or 10 slices for M–D, S–M, or S–D, respectively), or the combination of four antagonists (c, green, n = 12, 18, or 19 slices for M–D, S–M, or S–D, respectively), bicuculline (Bic), CGP, AP5, and 7Cl (for the comparison with 0, *p < 0.05, one-sample Wilcoxon signed rank test; for comparison between control and bicuculline, ^§p < 0.05, one-way ANOVA followed by the Fisher test)