Fig. 8
From: Ackr3-Venus knock-in mouse lights up brain vasculature
ACKR3-Venus vascular cellular expression in the adult mouse brain. Shown are representative images of brain sections from Ackr3Venus/Venus for ACKR3-Venus amplified expression (red) and vascular cell markers, vimentin (A, B) or CD31 (C–E) in cyan. Representative images show vimentin stains larger blood vessels in the cortex (CTX, A) and medulla (B). Anti-CD31 was used to mark endothelial cells (cyan) shown are representative images from the CTX (C), hippocampus (HPF, D) and cerebellum (CER, E) with amplified Venus. Images were taken on Olympus FV1200 Laser scanning confocal microscope 20 × objective. White arrowheads indicate cells that are co-labeled, A–E. Scale bar is A, B, 50 µm or C–E, 100 µm (1st column) and 20 µm (2nd–3rd columns)