Fig. 5
From: Genome-wide translation control analysis of developing human neurons
Translational Control of neuritogenesis, axon guidance, and cytoskeleton genes in Neurons. A IPA GO analysis of Actin cytoskeleton and neurite guidance processes enriched in translation-regulated genes. The dashed line marks FDR < 0.01. B RNAseq versus Riboseq FC plot of dendritic and axonal AMIGO GO processes genes regulated between neurons and NPCs. Different translation regulation categories are indicated in the figure. C TE heatmap of cytoskeleton regulators and structural genes. Data are presented as the TE fold-change value in Neurons compared to NPC. D CFL1, DPYSL2, and SS18L1 mRNA are strongly associated with heavy polysomes in neurons. The graphic represents an average of 3 independent experiments. Asterisks indicate **** p < 0.0001. E GPM6A and STMN3 are more induced at the protein level than at the mRNA level during the differentiation of NPCs into neurons. Comparative protein levels were determined by western blot, meanwhile mRNA levels were determined by qPCR. The analysis was performed in 4 independent biological samples